Test result should be interpreted as positive only when theīands include p24 and p31, and gp41 or gp120/160 (12) (see TableĬonversely, a negative Du Pont Western blot test result requiresĪbsence of any and all bands-not just viral-bands. The currently licensed Du Pont Western blot test specifies that the There has been substantial debate regarding the interpretive Specimens with only gp41 antibodies produced bands at the 41-,Īlthough the overall sensitivity and specificity of the Westernįor detection of antibodies to the various viral proteins are high, In fact, the true gp120 was shown to be absent from someĬommercial Western blot antigens. May have resulted in part from a reaction with a multimeric form of That the reaction observed against the gp120 on certain Western However, in a recent study, the protein with aġ60 kilodaltons (kd) present in commercially available WesternĪnd in viral lysate antigen preparations was identified as a Virtually all HIV-infected persons regardless of clinical stageĪntibodies to the polymerase (POL) gene products (p31, p51, andĪre also commonly detected if these antigens are present on theīlot strips. In contrast,Īntibodies to the envelope (ENV) precursor protein gp160 and theĮNV proteins (gp120 and gp41) can be detected in specimens from With onset or progression of clinical symptoms (4-9). Infection by Western blot and tend to decrease or become Protein p24, and its precursor p55, are the earliest detected after Antibodies to the HIV-1 major group-specific antigen (GAG) Table 1 lists the major structural proteins coded for by the HIV Negative control serum specimens are run simultaneously to allow Presence of substrate will produce a colored band.

Immunoglobulin G (IgG) antibody conjugated with an enzyme that in Any HIVĪntibody from the patient's serum is detected by an antihuman Nitrocellulose paper and reacted with the patient's serum. The viral proteins are then transferred onto HIV-1 lysate are separated according to size by polyacrylamide gelĮlectrophoresis. The Western blot assay is a method in which individual proteins of Results and their use in diagnosing HIV-1 infection. Report is to provide guidance for interpreting Western blot test Licensed by the Food and Drug Administration (FDA). To date, only one commercial Western blot test (Du PontPr) has More specific test, the most common of which is the Western blot In the EIA for HIV-1 antibody are then retested with a supplemental Seroprevalence surveys, and for blood-donor screening.Įnzyme immunoassay (EIA) is the most widely used serologic testĭetecting antibody to HIV-1. Including clinical diagnosis of HIV-1 infection-for symptomaticĪsymptomatic patients in counseling and testing programs-for These tests have been used for various purposes, Retrovirus was identified as the cause of acquired immunodeficiency Immunodeficiency virus type 1 (HIV-1) progressed rapidly after this The development of sensitive and specific tests for antibody to Reach an accurate diagnosis for persons tested for HIV-1 infection. Health policy officials with useful information in their efforts to Which to report results in order to provide clinicians and public Provides recommendations for use of the Western blot and the manner Specificity of these criteria as tools for public health practice, With the Western blot test for HIV-1, evaluates the sensitivity and It includes a description of various interpretive criteria The Association of State and Territorial Public Health Laboratoryĭirectors (ASTPHLD) and CDC have collaborated in preparing this Public Health Practice Program Office, Centers for Disease Control* Laboratory Directors and AIDS Program, Center for Infectious Reported by: Association of State and Territorial Public Health Please note: This guideline document is obsolete and may not reflect current evidence or best practice and likely contains out-of-date information. Interpretation and Use of the Western Blot Assayįor Serodiagnosis of Human Immunodeficiency Virus Type 1 Infections For assistance, please send e-mail to: Type 508 Accommodation and the title of the report in the subject line of e-mail. Persons using assistive technology might not be able to fully access information in this file.

Western blot procedure simple